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JoVE Journal Neuroscience
Visualisering Motor Axon navigasjon og kvantifisering av Axon Arborization i Mouse embryoer bruker lys ark fluorescens mikroskopi
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Visualisering Motor Axon navigasjon og kvantifisering av Axon Arborization i Mouse embryoer bruker lys ark fluorescens mikroskopi

Article DOI: 10.3791/57546-v • 08:56 min • May 11th, 2018
May 11th, 2018 •
*1,2, *2,3, 1,2
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, 2Institute of Molecular Biology, Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture, National Taiwan University
* These authors contributed equally

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Her beskriver vi en protokoll for å visualisere motoriske nervecellen projeksjon og axon arborization i transgene Hb9::GFP mouse embryoer. Etter immunostaining for motor neurons brukte vi lys ark fluorescens mikroskopi til bildet embryo for påfølgende kvantitativ analyse. Denne protokollen gjelder andre Nevron navigasjon prosesser i sentralnervesystemet.

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Nevrovitenskap problemet 135 Motor neuron axon navigasjon axon bane axon arborization lys ark mikroskopi ryggmargsskade IMARIS
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