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Uma abordagem Microfluidic gotículas e amplificação por PCR-microesfera para Single-Stranded DNA Amplicons
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
DOI:

11:40 min

November 14, 2018

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Chapters

  • 00:04Title
  • 01:35Fabrication of a PDMS Microfluidic Platform
  • 03:16Production of Polyacrylamide Oligo-Microspheres
  • 06:06Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
  • 07:06Microsphere-PCR for Amplifying ssDNA
  • 08:08Confocal Microscopy Acquisition
  • 08:38“Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification”
  • 10:33Conclusion

Summary

Automatic Translation

Este trabalho fornece um método para a fabricação de plataformas baseadas em gotículas microfluidic e a aplicação de microesferas de poliacrilamida para amplificação por PCR-microesfera. O método de PCR-microesfera torna possível obter single-stranded DNA amplicons sem separar o DNA de cadeia dupla.

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