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健康脑-垂体切片对硬骨鱼类鱼垂体细胞电生理的研究
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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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健康脑-垂体切片对硬骨鱼类鱼垂体细胞电生理的研究

DOI:
10.3791/57790-v

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07:14 min

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August 16, 2018

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, ,
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Chapters

  • 00:04Title
  • 00:47Tissue Harvest and Section Acquisition
  • 03:06Perforated Patch-clamp and Electrophysiological Recordings
  • 05:39Results: Representative Pituitary Cell Current Clamp Recordings
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了利用硬骨鱼类鱼鳉 (Oryzias latipes) 制作可行的脑-垂体组织切片的优化协议, 然后用膜片钳技术进行垂体细胞的电生理记录, 并穿孔补丁配置。

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