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En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation
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Neuroscience
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JoVE Journal Neuroscience
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

En invasiv metode for aktivering af musen Dentate Gyrus af højfrekvente Stimulation

DOI:
10.3791/57857-v

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12:26 min

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June 02, 2018

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,
1Department of Neurobiology,Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration,Nantong University

Chapters

  • 00:04Title
  • 01:06Electrode Implantation
  • 05:24High-Frequency Stimulation Delivery
  • 06:56Immunofluorescence Staining and Bromodeoxyuridine Labeling
  • 10:05Results: Distinct activation of neurogenesis in the DG in response to HFS in DG and PP
  • 11:46Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol viser, hvordan du konfigurerer en pålidelig HFS metode i mus. Neuroner i hele den hippocampus dentate gyrus stimuleres elektrisk af HFS direkte og indirekte in vivo. Neuronal aktivitet og molekylær signalering er undersøgt af c-fos og Notch1 immunfluorescent farvning, henholdsvis; neurogenese er kvantificeret ved bromodeoxyuridine mærkning assay.

Tags

High-frequency StimulationNeuronal ActivityNeurogenesisHippocampusDeep Brain StimulationMouse Dentate GyrusStereotactic SurgeryElectrode ImplantationBrain Disorders

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