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你在哪里切的问题: 眼地标性小鼠视网膜空间定位的解剖与分析指南
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks
DOI:

08:42 min

August 04, 2018

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Chapters

  • 00:04Title
  • 00:37Using the Superior Rectus Muscle Landmark to Identify Retinal Orientation
  • 02:36Using the Choroid Fissure Landmark to Identify Retinal Orientation
  • 04:06Labeling the S-opsin Gradient in the Mouse Retina
  • 07:02Results: Using the S-opsin Gradient to Identify All Four Poles of the Retina
  • 08:09Conclusion

Summary

Automatic Translation

该协议提供了一个全面的解剖和分析指南, 使用深眼地标, s-视蛋白免疫组化, Retistruct 和自定义代码, 以准确和可靠地定向孤立的小鼠视网膜在解剖空间。

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