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10:46 min
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June 02, 2018
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Dieses Protokoll beschreibt die erforderlichen Schritte zum Entwerfen und ausführen gemultiplexten Ausrichtung der Enhancer mit deaktivieren Fusionsproteins SID4X-dCas9-KRABBE, auch bekannt als Enhancer Störungen (Enhancer-i). Dieses Protokoll ermöglicht die Identifizierung von Enhancer, die Genexpression regulieren und erleichtert das Sezieren von Beziehungen zwischen Enhancer Regulierung ein gemeinsames Zielgen.
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Cite this Article
Carleton, J. B., Berrett, K. C., Gertz, J. Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines. J. Vis. Exp. (136), e57883, doi:10.3791/57883 (2018).
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