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June 02, 2018
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Summary
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Dit protocol beschrijft de stappen die nodig zijn voor het ontwerpen en uitvoeren multiplexed targeting van versterkers met de deactivering fusieproteïne SID4X-dCas9-KRAB, ook bekend als enhancer interferentie (Enhancer-i). Dit protocol stelt de identificatie van versterkers die regelen van genexpressie en vergemakkelijkt de dissectie van de relaties tussen de versterkers van regulering van een gemeenschappelijk doel-gen.
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Cite this Article
Carleton, J. B., Berrett, K. C., Gertz, J. Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines. J. Vis. Exp. (136), e57883, doi:10.3791/57883 (2018).
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