Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials

This method can answer key questions on how to decorate peptide amphiphiles to polyamines and on the use of octagonal protecting groups The main advantage of this technique is that you can generate hybrid peptide amphiphiles with unprotected chemical building blocks containing multiple reactive sides. Demonstrating the procedure will be Mehdi, Nathalia and Krishnaiah, laboratory students from the Conda Sheridan Laboratory. First, carefully weigh out 2-Chlorotrityl chloride resin place the resin in a fritted, medium porocity, synthesis vessel with 50 milliliter capacity.

Next, affix the synethesis vessel to a variable speed mechanical shaker and turn the vessel to a 45 degree angle to maximize agitation. Then add 15 milliliters of DCM to the resin. After allowing the resin beads to swell for 15 minutes, add eight equivalents of the desired polyamine to the resin, and allow it to react for five hours.