JoVE Journal
Genetics
Genetics
This content is Free Access.
哺乳類システムのクローン作成 CRISPR ガイド RNA
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Article
DOI:
10.3791/57998-v
•
06:48 min
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October 2nd, 2018
哺乳類システムのクローン作成 CRISPR ガイド RNA
October 2nd, 2018
•
Sathiji Nageshwaran*1,2, Alejandro Chavez*1,2,3, Nan Cher Yeo1,2, Xiaoge Guo1,2, Alissa Lance-Byrne1, Angela Tung1, James J. Collins1,4,5,6,7, George M. Church1,2
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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ここでは、シンプルで効率的と sgRNA のクローニングのコスト効果の高い方法の通りです。