JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
CRISPR Guide RNA Klonen für Säugetier-Systeme
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
CRISPR Guide RNA Cloning for Mammalian Systems
Please note that all translations are automatically generated.
Click here for the English version.
CRISPR Guide RNA Klonen für Säugetier-Systeme
DOI:
10.3791/57998-v
•
06:48 min
•
October 02, 2018
•
Sathiji Nageshwaran*
1,2
,
Alejandro Chavez*
1,2,3
,
Nan Cher Yeo
2
,
Xiaoge Guo
2
,
Alissa Lance-Byrne
,
Angela Tung
,
James J. Collins
4,5,6,7
,
George M. Church
2
1
Wyss Institute for Biologically Inspired Engineering
,
Harvard University
,
2
Department of Genetics
,
Harvard Medical School
,
3
Department of Pathology
,
Massachusetts General Hospital
,
4
Institute for Medical Engineering & Science
,
Massachusetts Institute of Technology
,
5
Synthetic Biology Center
,
Massachusetts Institute of Technology
,
6
Department of Biological Engineering
,
Massachusetts Institute of Technology
,
7
Broad Institute
Chapters
00:04
Title
00:25
Preparation of the Primers and Expression Vectors
02:05
Ligating the Annealed gRNA Oligonucleotides
02:59
Transformation of
E. coli
04:03
PCR Analysis
05:35
Results: CRISPR Guided RNA Cloning
06:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
Hier ist eine einfache, effiziente, und kosteneffektive Methode des Klonens SgRNA skizziert.
Tags
CRISPR
Guide RNA
Cloning
Mammalian System
Plasmid
Single-guide RNA
Oligonucleotide
Ligation
E. Coli
Competent Cells
Transformation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Zuordnung von RNA-RNA-Wechselwirkungen unter Verwendung von biotinyliertem Psoralen
Selektionsabhängige und unabhängige Erzeugung von CRISPR / Cas9-vermittelten Gen-Knockouts in Säugetierzellen
CRISPR-vermittelten Reorganisation der Chromatinstruktur mit Schleife
Seltenes Ereigniserkennung mit Fehler korrigiert DNA und RNA Sequenzierung
Endogene Protein Tagging in menschlichen induzierten pluripotenten Stammzellen mit CRISPR/Cas9
Eine anpassbare Protokoll für String Montage gRNA Klonen (STAgR)
CRISPR-vermittelten Genom-Bearbeitung von menschlichen pilzliche Erreger
Candida albicans
Genom-Bearbeitung in Säugetieren Zelllinien mit CRISPR-Cas
Künstliche RNA-Polymerase II-Dehnungskomplexe zur Sezieren von co-transkriptionellen RNA-Verarbeitungsereignissen
CRISPR/Cas9 Ribonucleoprotein-vermittelte präzise Genbearbeitung durch Tube Electroporation
Read Article