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人牙小泡干细胞的分离、鉴定及微 rna 遗传修饰
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Genetics
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JoVE Journal Genetics
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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人牙小泡干细胞的分离、鉴定及微 rna 遗传修饰

DOI:
10.3791/58089-v

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07:56 min

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November 16, 2018

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, , , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, 2Department Life,Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology,Rostock University Medical Center

Chapters

  • 00:04Title
  • 00:33Isolation of hDFSCs
  • 02:50Transfection of hDFSCs
  • 04:07Analysis of Transfection
  • 06:02Results: Successful Isolation and Genetic Modification of hDFSC
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了从人毛囊中提取的牙干细胞的瞬态基因工程。应用的非病毒性修饰策略可以为治疗性干细胞产品的改进奠定基础。

Tags

Dental Follicle Stem CellsHDFSCsEnzymatic DigestionCollagenaseDispaseMicroRNAGenetic ModificationRegenerative MedicineCell IsolationCell Characterization

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