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Biochemistry

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Funktionelle karakterisering af Carboxylesterases i insekticid resistente stuefluer, Musca Domestica
 
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Funktionelle karakterisering af Carboxylesterases i insekticid resistente stuefluer, Musca Domestica

Article DOI: 10.3791/58106
August 23rd, 2018

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Summary August 23rd, 2018

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Vi præsenterer her, en protokol for at producere hus flyve carboxylesterase proteiner i vitro med en baculovirus medieret insekt celle ekspressionssystem og senere funktionelt karakteriserer deres roller i metaboliske permethrin, dermed overdrage pyrethroid modstand af celle-baseret MTT assay og in vitro- metaboliske undersøgelser.

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