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Carboxylesterases 在抗杀虫剂家蝇、家蝇中的功能表征
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
DOI:

08:17 min

August 23, 2018

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Chapters

  • 00:04Title
  • 00:53Expression and Isolation of Target Proteins with a Baculovirus-mediated Insect Cell Expression System
  • 04:18A Cell-based MTT Cytotoxicity Assay
  • 06:30Results: MdαE7 Expressing Cells Show Higher Viability when Exposed to Permethrin
  • 07:12Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 生产的房子飞羧酸蛋白的体外与杆状病毒介导的昆虫细胞表达系统, 后来功能表征其在代谢氯菊酯的作用, 从而, 赋予以传导细胞为基础的 mtt 法和体外代谢研究的抗性。

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