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Neuroscience
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耦合星形胶质细胞网络的大小、形状和方向性分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
Please note that all translations are automatically generated.
Click here for the English version.
耦合星形胶质细胞网络的大小、形状和方向性分析
DOI:
10.3791/58116-v
•
10:10 min
•
October 04, 2018
•
Steven Condamine
,
Dorly Verdier
,
Arlette Kolta
2
1
Groupe de Recherche sur le Système Nerveux Central, and Département de Neurosciences
,
Université de Montréal
,
2
Faculté de Médecine Dentaire
,
Université de Montréal
Chapters
00:04
Title
00:30
Data Preparation
02:32
Cell Counting
04:10
Measuring Astrocytic Network Area
05:04
Determining the Main Direction Vector
06:52
Placing the Analyzed Networks in the Nucleus of Interest
09:11
Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
09:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个评估胶质网络组织的协议。所述方法最大限度地减少偏倚, 以提供这些网络的描述性度量, 如细胞计数、大小、面积和原子核内的位置。各向异性的评估与矢量分析。
Tags
Astrocytic Networks
Astrocytes
FIJI
ImageJ
Z Stack
Max Intensity Projection
Background Subtraction
Noise Reduction
Binary Image
Cell Counting
Morphology Analysis
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