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Analysere størrelse, form og retning for indtastning af netværk af koblede astrocytter
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
DOI:

10:10 min

October 04, 2018

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Chapters

  • 00:04Title
  • 00:30Data Preparation
  • 02:32Cell Counting
  • 04:10Measuring Astrocytic Network Area
  • 05:04Determining the Main Direction Vector
  • 06:52Placing the Analyzed Networks in the Nucleus of Interest
  • 09:11Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
  • 09:49Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol for at vurdere organiseringen af astrocytic netværk. Den beskrevne metode minimerer bias for at give beskrivende foranstaltninger af disse netværk såsom celletal, størrelse, område og position inden for en kerne. Anisotropy vurderes med en vektorielle analyse.

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