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Journal
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Biochemistry
/
开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
Please note that all translations are automatically generated.
Click here for the English version.
开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示
DOI:
10.3791/58228-v
•
05:42 min
•
January 07, 2019
•
Jae Gon Kim*
1
,
Sang Woong Park*
2
,
Kyung Chul Shin
,
Bokyung Kim
,
Doyoung Byun
,
Young Min Bae
1
Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science
,
Konkuk University School of Medicine
,
2
Department of Emergency Medical Services
,
Eulji University
,
3
Department of Mechanical Engineering
,
Sungkyunkwan University
Chapters
00:04
Title
00:30
Agarose Salt Bridges Between the Bath Solution and Reference Electrode
01:29
Application of Fluid Flow Shear Force to Cells in a Patch-Clamping Chamber
02:21
Measuring Changes in Liquid-Metal Junction Potential
03:18
Experimental Estimation of Real Chloride Concentration
04:07
Results: Effects of Fluid Flow on L-type VDCCL and Kir2.1 Currents With and Without Agarose 3 M KCl Bridge
05:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
利用膜片钳记录, 从流体流动剪切力敏感性的角度研究了机械敏感离子通道。然而, 根据实验方案, 离子通道流体流动规律的结果可能是错误的。在这里, 我们提供了防止和纠正此类错误的方法, 并提供了理论依据。
Tags
Ion Concentration
Unstirred Boundary Layer
Open Patch-clamp Pipette
Fluid Flow
Ion Channels
Agarose
Potassium Chloride
Junction Potential
Voltage Clamp
Current Clamp
Bathing Solution
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