JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
在培养的小鼠神经元中可视化轴突生长锥塌陷和早期淀粉样β效应
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons
Please note that all translations are automatically generated.
Click here for the English version.
在培养的小鼠神经元中可视化轴突生长锥塌陷和早期淀粉样β效应
DOI:
10.3791/58229-v
•
06:23 min
•
October 30, 2018
•
Tomoharu Kuboyama
1
Division of Neuromedical Science, Institute of Natural Medicine
,
University of Toyama
Chapters
00:04
Title
00:35
Neuron Culture
02:34
Growth Cone Collapse Assay
04:27
Results: Aß1-42-induced Axonal Growth Cone Collapse
05:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了一种研究淀粉样蛋白β (Aβ) 在脑内早期作用的实验方案。 这表明 Aβ诱导网格蛋白介导的内吞和轴突生长锥的崩溃。该协议有助于研究 Aβ对轴突生长锥的早期影响, 并可促进预防阿尔茨海默病。
Tags
Axonal Growth Cone
Amyloid Beta
Alzheimer’s Disease
Neuron Culture
Embryonic Mouse
Trypsin
DNAse
Soybean Trypsin Inhibitor
Cell Filtration
Hemocytometer
Cell Density
Aggregated Amyloid Beta 1-42
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
机械操纵控制神经元轴突的发展
在培养的中枢神经系统的神经元树突棘形态分析
transretinal ERG的录音，从小鼠视网膜杆和锥光反应
诱导培养的交感神经元的树突状增长
成像pHluorin标记插入到质膜受体原代培养的小鼠神经元
可视化的一个积极的早期经验，触觉刺激的影响，对树突状形态和突触连接与高尔基染色考克斯
初级量子神经元轴突运输的实时成像点标记的BDNF
嗅觉系统作为模型来研究轴突生长的模式和形态<em>在体内</em
低聚淀粉样蛋白-β的立体灌注到小鼠海马
使用荧光探针定量培养的神经元中的内体和溶酶体运动
Read Article
