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Journal
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Bioengineering
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高通量自动 Microbioreactor 系统在 CHO 细胞模型 IgG1 生产中的应用
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
Please note that all translations are automatically generated.
Click here for the English version.
高通量自动 Microbioreactor 系统在 CHO 细胞模型 IgG1 生产中的应用
DOI:
10.3791/58231-v
•
08:15 min
•
September 28, 2018
•
Sai Rashmika Velugula-Yellela
,
Casey Kohnhorst
,
David N. Powers
,
Nicholas Trunfio
,
Anneliese Faustino
,
Phillip Angart
,
Erica Berilla
,
Talia Faison
,
Cyrus Agarabi
1
Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II
,
U.S. Food and Drug Administration
Chapters
00:04
Title
00:46
Seed Train Expansion and Automated Microbioreactor System Preparation
02:55
Automated Microbioreactor Software Initiation
05:15
System Shutdown
05:58
Cell Culture Harvest and IgG Titer Measurement
07:04
Results: Representative High-Throughput Automated Microbioreactor Analyses
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种 microbioreactor 系统在不同条件下并行运行48平行细胞培养的详细协议。介绍了细胞培养过程、收获和随后的抗体效价分析。
Tags
High-throughput
Automated Microbioreactor System
IgG1 Production
CHO Cells
Cell Line Characterization
Process Optimization
Shake Flasks
Automation
Small-scale Studies
Cell Culture
Subculturing
Cell Counter
Microbioreactor Software
Temperature Control
Dissolved Oxygen
PH Monitoring
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