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Arbovirus infeksjoner som Screening verktøy for identifikasjon av Viral Immunomodulators og vert Antiviral faktorer
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
DOI:

06:02 min

September 13, 2018

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Chapters

  • 00:04Title
  • 00:48General Lymantria dispar (LD652) Cell and Virus Culture
  • 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
  • 02:43Luciferase Assay
  • 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
  • 05:04Conclusion

Summary

Automatic Translation

Her presenterer vi protokoller for å identifisere 1) virus-kodede immunomodulators som fremmer arbovirus replikering og 2) eukaryote vert faktorer som begrenser arbovirus replikering. Metodene fluorescens og luminescence-baserte tillate forskere å raskt få kvantitative readouts arbovirus replikasjon i enkle analyser med lav signal-til-støy-forhold.

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