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Immunhistokemisk påvisning af 5-Methylcytosine og 5-Hydroxymethylcytosine i at udvikle og Postmitotic mus nethinden
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
DOI:

07:50 min

August 29, 2018

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Chapters

  • 00:04Title
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

Summary

Automatic Translation

Formålet med denne rapport er at beskrive protokollen for robust immunhistokemisk påvisning af epigenetiske markører, 5-methylcytosine (5mC) og 5-hydroxymethylcytosine (5hmC) i at udvikle og postmitotic musen nethinden.

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