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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
 
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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究

Article DOI: 10.3791/58323
October 23rd, 2018

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Summary October 23rd, 2018

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在这里, 我们提出了一个分离健康和功能的原发性小鼠肝细胞的协议。通过非放射性标记基底检测肝新生蛋白合成的说明, 帮助了解肝脏能量代谢稳态背景下蛋白质合成的机理。

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