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新生儿初乳喂养前后离散催产素分泌脑核细胞应激与炎症的评估
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
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新生儿初乳喂养前后离散催产素分泌脑核细胞应激与炎症的评估

DOI:
10.3791/58341-v

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09:12 min

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November 14, 2018

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, , , , , ,
1Department of Psychiatry,Columbia University, 2Department of Pathology & Cell Biology,Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology,New York State Psychiatric Institute, 4EB Sciences

Chapters

  • 00:04Title
  • 00:48Tissue Preparation and Protein Extraction
  • 02:46In-capillary Protein Measurement Sample Preparation and Signal Protein Analysis
  • 06:32Results: Representative Signaling Protein Marker Expression in Primed and Unprimed Neonatal Rat Brain Tissue
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个方案, 以分离脑核在新生大鼠大脑与第一初乳喂养。这项技术允许研究由肠细胞信号调节的大脑营养功能不足应激。

Tags

OxytocinBrain NucleiNeonatal RatColostrum FeedingCellular StressInflammationPost-natal DevelopmentMilk SucklingBrain PunchesProtein ExtractionProtein Measurement

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