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福尔马林固定癌组织标本免疫肿瘤学自动复合免疫荧光小组研究
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Cancer Research
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JoVE Journal Cancer Research
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
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福尔马林固定癌组织标本免疫肿瘤学自动复合免疫荧光小组研究

DOI:
10.3791/58390-v

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10:49 min

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January 21, 2019

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, , , , , , , , , , , , , ,
1Laboratory of Pathology,MedImmune, 2Laboratory of Pathology,MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology,University of Texas – MD Anderson Cancer Center

Chapters

  • 00:04Title
  • 01:17Multiplex Immunofluorescence (mIF) Protocol Program Creation
  • 03:09Reagent Preparation
  • 05:19Multispectral Imaging
  • 06:45Spectral Unmixing, Fluorescence Intensity, and Signal Attenuation
  • 08:15Results: Representative mIF Imaging
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

对6个标记多重免疫荧光面板的详细协议进行了优化和执行, 使用自动着色器实现更一致的结果和更短的手术时间。这种方法可以直接由任何实验室用于免疫肿瘤学研究。

Tags

Multiplex ImmunofluorescenceImmuno-oncologyAutomated StainingProtocol OptimizationImmune Cell PhenotypingPrognostic And Predictive BiomarkersSpectral ImagingPeroxidase InhibitionPrimary And Secondary AntibodiesTyramide Signal AmplificationAntigen RetrievalDrop ControlsIsotype Controls

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