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Journal / Neuroscience / 成人脊髓细胞核在大规模平行单核 RNA…
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JoVE Journal Neuroscience
成人脊髓细胞核在大规模平行单核 RNA 测序中的分离
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成人脊髓细胞核在大规模平行单核 RNA 测序中的分离

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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在这里, 我们提出了一个协议, 快速隔离高品质的核从新鲜或冷冻组织的下游大规模平行 RNA 测序。我们包括洗涤剂-机械和低渗-机械组织破坏和细胞裂解选择, 两者都可用于核分离。

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神经科学 问题 140 细胞核 RNA 测序 snRNA 序列 大规模平行 脊髓 蔗糖梯度
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