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使用荧光激活的细胞核分选从新鲜冷冻的皮质中分离出成年人类星形胶质细胞群
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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使用荧光激活的细胞核分选从新鲜冷冻的皮质中分离出成年人类星形胶质细胞群

DOI:
10.3791/62405-v

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08:18 min

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April 16, 2021

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, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Introduction
  • 00:42Frozen Tissue Dissociation and Ultracentrifugation
  • 02:13Fluorescence-Activated Nuclei Sorting (FANS)
  • 04:32Downstream Molecular Analyses
  • 05:58Results: Analysis of Human Astrocyte Populations after FANS
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

我们开发了一种从新鲜冷冻组织中富集和分离人类星形胶质细胞群的方法，用于下游分子分析。

Tags

Adult Human AstrocyteFluorescence-activated Nuclei SortingFresh-frozen CortexCell IsolationCell Type EnrichmentNeuronOligodendrocyte Progenitor CellSucrose BufferUltracentrifugationNuclei ResuspensionTrypan BlueFluorescence-activated Cell SortingNeuNPAX6DAPIForward ScatterSide ScatterSinglet Nuclei

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