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Bioengineering
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多平行板流室对剪切应力暴露的内皮细胞基因表达分析
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Bioengineering
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JoVE Journal
Bioengineering
Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
Please note that all translations are automatically generated.
Click here for the English version.
多平行板流室对剪切应力暴露的内皮细胞基因表达分析
DOI:
10.3791/58478-v
•
08:50 min
•
October 21, 2018
•
H.S. Jeffrey Man
2
,
Aravin N. Sukumar
2
,
Kyung Ha Ku
3
,
Michelle K. Dubinsky
2
,
Noeline Subramaniam
2
,
Philip A. Marsden
2,3,4
1
Institute of Medical Science
,
University of Toronto
,
2
Keenan Research Centre in the Li Ka Shing Knowledge Institute
,
St. Michael’s Hospital
,
3
Department of Laboratory Medicine and Pathobiology
,
University of Toronto
,
4
Department of Medicine
,
University of Toronto
Chapters
00:04
Title
00:55
Assay Setup
04:20
Flow Assay
05:18
Cell Harvest and RNA Extraction
06:48
Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
08:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了流体剪切应力作用下内皮细胞培养和基因表达分析的工作流程。包括一个物理安排, 可同时在受控环境中同时外壳和监测多个流室, 并使用外源参考 RNA 进行定量 PCR。
Tags
Endothelial Cells
Shear Stress
Parallel-plate Flow Chambers
Gene Expression Analysis
Atherosclerosis
Laminar Flow
Cell Culture
Fibronectin
CO2 Incubator
Bubble Trap
Cell Seeding
Tubing
Stopcock
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