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联合遗传和化学衣壳改性腺病毒基因转移载体用于屏蔽和靶向
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JoVE Journal Biology
Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
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联合遗传和化学衣壳改性腺病毒基因转移载体用于屏蔽和靶向

DOI:
10.3791/58480-v

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08:14 min

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October 26, 2018

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, ,
1Center of Biomedical Education and Research,University Witten/Herdecke

Chapters

  • 00:04Title
  • 01:31Coupling Moieties: Storage and Preparation
  • 02:43Amplification, Purification, and Chemical Modification of Ad Vectors
  • 06:36Results: Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

本文所述的协议使研究人员能够通过简单的化学方法在选定的地点专门修改腺病毒衣壳。可生成屏蔽腺病毒载体颗粒和重定目标基因转移载体, 并可研究载体宿主相互作用。

Tags

Gene TherapyOncolysisGenetic VaccinationAdenovirusGene Transfer VectorCapsid ModificationChemical ModificationVector-host InteractionVirus LabelingVirus Tracking

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