Journal
/
Medicine
/
Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery

Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery

DOI:
10.3791/58485-v

•

05:44 min

•

November 14, 2018

•

, , ,
1Laboratory of Pharmaceutics, School of Pharmacy,Nihon University

Chapters

  • 00:04Title
  • 01:04Intranasal Drug Administration
  • 03:11Radio-Labeled Water-Soluble Macromolecules ([14C]-inulin) Quantification
  • 04:32Results: Comparison of Brain [14C]-Inulin Levels After Two Different Methods of Intranasal Administration
  • 05:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe two novel methods of stable intranasal administration under inhalation anesthesia with minimal physical stress for experimental animals. We also describe a method for quantitative evaluation of drug distribution levels in the brain via the nose-to-brain pathway using radiolabeled [14C]-inulin as a model substrate of water-soluble macromolecules.

Tags

Keywords: Intranasal AdministrationNose-to-brain Drug DeliveryInhalation AnesthesiaCarbon-14 InulinReverse CannulationEsophageal CannulaProgrammable Microsyringe PumpBlood-brain BarrierPharmacokineticsPharmacologyCentral Nervous System DiseasesDrug Delivery Technologies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58485?article_type=v

A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation
check_url/58485?article_type=v

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
check_url/58485?article_type=v

Intranasal Administration of CNS Therapeutics to Awake Mice
check_url/58485?article_type=v

Heterotopic Mucosal Engrafting Procedure for Direct Drug Delivery to the Brain in Mice
check_url/58485?article_type=v

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium
check_url/58485?article_type=v

Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model
check_url/58485?article_type=v

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
check_url/58485?article_type=v

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
check_url/58485?article_type=v

CO2-Lasertonsillotomy Under Local Anesthesia in Adults
check_url/58485?article_type=v

Intratracheal Administration of Dry Powder Formulation in Mice

Read Article