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Chapters
- 00:05Title
- 01:52Synthesis of Mercury Cadmium Selenide (HgxCd1-xSe) Quantum Dot Cores
- 06:49Growth of Cadmium Zinc Sulfide (CdyZn1-yS) Shell
- 09:20Phase Transfer
- 12:01PEG Coating
- 13:10Results: Quantum Dot Characterization
- 16:39Conclusion
We describe the preparation of colloidal quantum dots with minimized hydrodynamic size for single-molecule fluorescence imaging. Compared to conventional quantum dots, these nanoparticles are similar in size to globular proteins and are optimized for single-molecule brightness, stability against photodegradation, and resistance to nonspecific binding to proteins and cells.
Tags
Compact Quantum DotsSingle-molecule ImagingBiomolecular FunctionSpatial HeterogeneityTemporal HeterogeneityEpifluorescence MicroscopyTotal Internal Reflection Fluorescence MicroscopyFluorescent TagDyeProteinBeadQuantum DotHigh Photon FluxesFluorescence ImagingSignal StabilityHydrodynamic SizeDiffusion BehaviorSemiconductor MaterialsPhotodegradation
