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Samtidige studie av rekrutteringen av Monocyte subpopulasjoner Under flyt In Vitro
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1Hematology service, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva
Chapters
- 00:04Title
- 00:54Human Umbilical Vein Endothelial Cell (HUVEC) Staining and Human Pan Monocyte Isolation
- 03:20Fluidic System Preparation
- 05:04Time-lapse Imaging of Monocyte Recruitment Under Flow and Image Analysis
- 06:56Results: Representative Monocyte Transmigration Analysis
- 08:25Conclusion
Her presenterer vi en integrert protokoll som måler monocytt subpopulasjon smugling under flyt i vitro ved bruk av bestemte overflate markører og AC confocal fluorescens mikroskopi. Denne protokollen kan brukes å utforske sekvensiell rekruttering trinn også om profil andre leukocytter subtyper med andre spesifikke overflate markører.
