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Biochemistry
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用核磁共振 (nmr) 和微尺度热能 (mst) 测量球状和丝状蛋白的相互作用
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
Please note that all translations are automatically generated.
Click here for the English version.
用核磁共振 (nmr) 和微尺度热能 (mst) 测量球状和丝状蛋白的相互作用
DOI:
10.3791/58537-v
•
10:28 min
•
November 02, 2018
•
Graeden Winkelaar
,
Catharine Trieber
,
Jitendra Kumar
,
Michael Overduin
1
Department of Biochemistry
,
University of Alberta
Chapters
00:04
Title
00:24
NMR Experimental Setup
04:25
NMR Data Processing
05:47
NMR Data Analysis
08:13
Results: Vimentin 99-249 (VimRod) Binds Wildtype Envoplakin Plakin Repeat Domain (E-PRD) but not Mutant R1914E E-PRD
09:54
Conclusion
Summary
Automatic Translation
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在这里, 我们提出了一个协议, 生产和纯化的蛋白质标记与稳定同位素, 以及随后的表征蛋白质相互作用使用核磁共振 (nmr) 光谱和微尺度热像仪 (mst) 实验。
Tags
Protein Interaction
NMR Spectroscopy
Microscale Thermophoresis
MST
Atomic-level Interaction
Protein Binding
Small Molecule
Large Molecule
Proton NMR
SOFAST HMBC
Magnetic Field Shimming
Receiver Gain Adjustment
Automatic Tuning
Phase Correction
Baseline Correction
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