/

/

可视化和跟踪活果蝇黑色素细胞卵室的内源性 mRNA

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Please note that all translations are automatically generated. Click here for the English version.

可视化和跟踪活果蝇黑色素细胞卵室的内源性 mRNA

DOI:

10.3791/58545-v

•

07:39 min

•

June 04, 2019

•

Irina E. Catrina, Livia V. Bayer², Omar S. Omar², Diana P. Bratu²

¹Biological Sciences Department Hunter College,City University of New York, ²Program in Molecular, Cellular, and Developmental Biology,Graduate Center, City University of New York

Chapters

00:04Title
00:51Molecular Beacon (MB) Nurse Cell Microinjection
02:38Tracking and Colocalization Analyses and Video File Preparation
05:58Results: Representative MB Visualization and Tracking
07:15Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,用于可视化,检测,分析和跟踪内源性mRNA贩运在活的果蝇黑色素细胞卵室使用分子信标,旋转盘共聚焦显微镜,和开源分析软件。

Tags

MRNA Drosophila Melanogaster Egg Chambers RNA Localization Live Imaging Molecular Beacons Microinjection RNA Biology Real-time Visualization RNA Trafficking Therapeutic Targets Zebrafish Cell Culture Microscopy Image Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在果蝇幼虫中枢神经系统的剖析

高分辨率视频跟踪移动的，在成年果蝇。

可视化的Live果蝇胶质细胞神经肌肉交界处，与荧光染料

可视化心脏跳动果蝇</em

实时成像果蝇胚胎血细胞迁移

分离果蝇睾丸

准备个人果蝇蛋钱伯斯实时成像

寿命测量<em>果蝇</em

凋亡细胞清除过程中实时成像<em>果蝇</em>胚胎发育

的维持<em>果蝇</em>人口笼

Read Article

JoVE

Privacy  •  Terms of Use  •  Policies

Share on Twitter

Share on Facebook

Share on LinkedIn

Share on Wechat

Contact Us

Recommend to library

JoVE NEWSLETTERS

RESEARCH

JoVE Journal

Methods Collections

JoVE Encyclopedia of Experiments

Archive

EDUCATION

JoVE Core

JoVE Business

JoVE Science Education

JoVE Lab Manual

Faculty Resource Center

Faculty Site

Authors

Overview

Publishing Process

Editorial Board

Scope and Policies

Peer Review

FAQ

Submit

Librarians

Overview

Testimonials

Subscriptions

Access

Resources

Library Advisory Board

FAQ

ABOUT JoVE

Overview

Leadership

Blog

JoVE

Privacy  •  Terms of Use  •  Policies

Share on Twitter

Share on Facebook

Share on LinkedIn

Share on Wechat

Contact Us

Recommend to library

JoVE NEWSLETTERS

RESEARCH

JoVE Journal

Methods Collections

JoVE Encyclopedia of Experiments

Archive

EDUCATION

JoVE Core

JoVE Business

JoVE Science Education

JoVE Lab Manual

Faculty Resource Center

Faculty Site

Authors

Overview

Publishing Process

Editorial Board

Scope and Policies

Peer Review

FAQ

Submit

Librarians

Overview

Testimonials

Subscriptions

Access

Resources

Library Advisory Board

FAQ

ABOUT JoVE

Overview

Leadership

Blog

RESEARCH

JoVE Journal

Methods Collections

JoVE Encyclopedia of Experiments

Archive

EDUCATION

JoVE Core

JoVE Business

JoVE Science Education

JoVE Lab Manual

Faculty Resource Center

Faculty Site

Authors

Overview

Publishing Process

Editorial Board

Scope and Policies

Peer Review

FAQ

Submit

Librarians

Overview

Testimonials

Subscriptions

Access

Resources

Library Advisory Board

FAQ

ABOUT JoVE

Overview

Leadership

Blog

JoVE

Privacy  •  Terms of Use  •  Policies

Share on Twitter

Share on Facebook

Share on LinkedIn

Share on Wechat

Contact Us

Recommend to library

JoVE NEWSLETTERS

Browse...

Research

Education

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Authors

Overview Publishing Process Editorial Board Scope and Policies Peer Review FAQ Submit

Librarians

Overview Testimonials Subscriptions Access Resources Library Advisory Board FAQ

ABOUT JoVE

Overview Leadership Blog

Share on Twitter

Share on Facebook

Share on LinkedIn

Share on Wechat

Contact Us

Recommend to library

JoVE NEWSLETTERS

Privacy

.

Terms of Use

.

Policies

Copyright © 2024 MyJoVE Corporation. All rights reserved

Copyright © 2024 MyJoVE Corporation. All rights reserved

-1::1