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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
 

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Article DOI: 10.3791/58612
October 25th, 2018

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Summary October 25th, 2018

Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples. 

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