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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

DOI:
10.3791/58612-v

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06:12 min

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October 25, 2018

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Chapters

  • 00:04Title
  • 00:30Sample Preparation
  • 01:39Immunomagnetic Separation (IMS) and Multiple Displacement Amplification (MDA)
  • 03:37Real-time PCR and Library Preparation for Quasimetagenomic Sequencing
  • 04:52Results: RT-PCR Amplifies DNA from Two Brands of Salmonella Contaminated Chicken Breast
  • 05:19Conclusion

Summary

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Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples. 

Tags

Keywords: SalmonellaQuasi-metagenomic AnalysisFood SampleEnvironmental SamplePathogen DetectionGenetic FingerprintingRV Enrichment BrothCentrifugationAnti-Salmonella BeadsMagnetic SeparationMultiple Displacement Amplification (MDA)

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