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Journal / Immunology and Infection / Automatiseret adfærdsmæssige analyse af store C.…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Automatiseret adfærdsmæssige analyse af store C. elegans befolkninger ved hjælp af et bredt synsfelt sporer Platform
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Automatiseret adfærdsmæssige analyse af store C. elegans befolkninger ved hjælp af et bredt synsfelt sporer Platform

Article DOI: 10.3791/58643-v • 07:20 min • November 28th, 2018
November 28th, 2018 •
1, 1, 1, 2, 1, 1, 1
1Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, 2European Research Institute for the Biology of Aging, University Medical Centre Groningen

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Vi beskriver protokoller for at bruge den brede field-of-view nematodeprøveudtagning sporing platform (WF-NTP), som giver høj overførselshastighed fænotypiske karakterisering af store populationer af Caenorhabditis elegans. Disse protokoller kan bruges til at karakterisere subtile adfærdsmæssige ændringer i mutantstammen eller svar til farmakologisk behandling i en yderst skalerbar mode.

Tags

Immunologi og infektion sag 141 Drug discovery fænotype-baseret screening høj-throughput screening C. elegans amyloid dannelse Alzheimers sygdom neurodegeneration ødelægge bibliotek stor befolkning analyse
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