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Visualisering af bakterielt toksin Inducerede Responses Brug af Live Cell Fluorescence Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
DOI:

14:29 min

October 01, 2012

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Chapters

  • 00:05Title
  • 01:09Preparation of Streptolysin O
  • 05:22Cell Lytic Assay
  • 06:44Micropipette Delivery of Toxin to Cultured Cells
  • 10:24Representative Purification and Imaging Results
  • 13:56Conclusion

Summary

Automatic Translation

Fremgangsmåder til oprensning af cholesterol binding toksin streptolysin O fra rekombinant<em> E. coli</em> Og visualisering af toxin binding til levende eukaryote celler er beskrevet. Lokal afgivelse af toksin inducerer hurtige og komplekse ændringer i målceller afslører hidtil ukendte aspekter af toksin biologi.

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