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Visualisering af bakterielt toksin Inducerede Responses Brug af Live Cell Fluorescence Microscopy
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Chapters
- 00:05Title
- 01:09Preparation of Streptolysin O
- 05:22Cell Lytic Assay
- 06:44Micropipette Delivery of Toxin to Cultured Cells
- 10:24Representative Purification and Imaging Results
- 13:56Conclusion
Fremgangsmåder til oprensning af cholesterol binding toksin streptolysin O fra rekombinant<em> E. coli</em> Og visualisering af toxin binding til levende eukaryote celler er beskrevet. Lokal afgivelse af toksin inducerer hurtige og komplekse ændringer i målceller afslører hidtil ukendte aspekter af toksin biologi.
Tags
VisualizationBacterial ToxinLive Cell Fluorescence MicroscopyMembranePoresLeakageCellular ContentsInfluxToxin ExposureCell TypeInflammatory ResponsesImmune CellsMacrophagesPro-inflammatory CytokinesGram Positive BacteriaVirulence MechanismsMorphologic ChangesPlasma MembraneCholesterol-enriched Surface ProtrusionsExtracellular SpaceCellular Defense MechanismEM (electron Microscopy)Chemical FixationImmune CellsMacrophagesInflammationMembrane VesiclesIL-1 Family Cytokines
