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Immunology and Infection
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建立与艾滋病毒相关的肝脏发病机制的双人性化TK-NOG小鼠模型
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Immunology and Infection
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
Please note that all translations are automatically generated.
Click here for the English version.
建立与艾滋病毒相关的肝脏发病机制的双人性化TK-NOG小鼠模型
DOI:
10.3791/58645-v
•
00:10 min
•
September 11, 2019
•
Raghubendra Singh Dagur
,
Weimin Wang
,
Edward Makarov
,
Yimin Sun
,
Larisa Y. Poluektova
1
Department of Pharmacology and Experimental Neuroscience
,
University of Nebraska Medical Center
,
2
Department of Pathology and Microbiology
,
University of Nebraska Medical Center
Chapters
00:04
Title
00:47
Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
04:25
Preparation of Human Hepatocytes for Transplantation
05:10
Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
06:50
HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
07:48
Results: Analysis of the Dual Humanized TK-NOG Mouse Model
09:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了一种可靠的方法,建立人类免疫系统和肝细胞的人性化小鼠。
通过
注射人肝细胞和CD34实现的双重组免疫缺陷小鼠
–
造血干细胞易受人体免疫缺陷病毒-1感染和重述肝损伤的影响,如艾滋病毒感染者。
Tags
HIV
Liver Pathogenesis
Humanized Mouse Model
Hematopoietic Stem Cells
Hepatocytes
Umbilical Cord Blood
CD34 Positive Cells
Cell Separation
Magnetic Activated Cell Sorting (MACS)
Cell Counting
Cell Culture
Transplantation
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