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Journal
/
Cancer Research
/
生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
Please note that all translations are automatically generated.
Click here for the English version.
生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统
DOI:
10.3791/58672-v
•
10:44 min
•
August 09, 2019
•
Raul Vizcardo*
1,2
,
S.M. Rafiqul Islam*
1,2
,
Takuya Maeda
2
,
Naritaka Tamaoki
2
,
Meghan L. Good
2
,
Nicholas D. Klemen
2
,
Marta Bosch-Marce
2
,
Li Jia
,
Mikhael J. Kruhlak
,
Nicholas P. Restifo
2
1
Surgery Branch
,
National Cancer Institute, NIH
,
2
Center for Cell-Based Therapy
,
National Cancer Institute, NIH
,
3
Laboratory of Cancer Biology and Genetics, Center for Cancer Research
,
National Cancer Institute, NIH
Chapters
00:04
Title
00:57
Preparation of OP9/DLL1 Cells for Co-culture with iPSC
02:08
In Vitro Differentiation of iPSC into Immature T Cells
05:00
3D Thymic Organ Culture to Generate iTE
08:00
Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
09:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种通过三维(3D)胸腺培养系统生成肿瘤抗原特异性诱导多能干细胞衍生胸腺(iTE)的新方法。iTE 是 T 细胞的同质子集,与天真的 T 细胞密切相关,具有增殖、记忆形成和肿瘤抑制的能力。
Tags
3D Thymic Culture
Induced Pluripotent Stem Cells
Tumor Antigen-specific T Cells
Thymic Emigrants
OP9/DLL1 Cells
Trypsinization
Cell Suspension
Cell Culture
T Cell Differentiation
In Vivo T Cell Generation
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