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一种研究受体配体相互作用的 bw 报告系统
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A BW Reporter System for Studying Receptor-Ligand Interactions
Please note that all translations are automatically generated.
Click here for the English version.
一种研究受体配体相互作用的 bw 报告系统
DOI:
10.3791/58685-v
•
06:05 min
•
January 07, 2019
•
Shlomo Elias
2
,
Shira Kahlon
,
Alexandra Duev-Cohen
,
Ofer Mandelboim
1
Department of Hematology
,
Hadassah – Hebrew University Medical Center
,
2
The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC)
,
Hadassah – Hebrew University Medical Center
Chapters
00:04
Title
00:36
Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells
03:10
Incubation of the Transfected BW Cells with Targets
03:32
ELISA
04:38
Results: BW Reporter System Demonstrated
05:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何建立一个可用于识别和量化受体配体相互作用的记者系统。
Tags
BW Reporter System
Receptor-ligand Interactions
Ligand
Endogenous Ligand
Transfection
PcDNA3 Plasmid
Sodium Acetate
Ethanol Precipitation
Electroporation
G418 Selection
96-well Plate
ELISA
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