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A BW Reporter System for Studying Receptor-Ligand Interactions
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JoVE Journal Biology
A BW Reporter System for Studying Receptor-Ligand Interactions

A BW Reporter System for Studying Receptor-Ligand Interactions

DOI:
10.3791/58685-v

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06:05 min

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January 07, 2019

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, , ,
1Department of Hematology,Hadassah – Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah – Hebrew University Medical Center

Chapters

  • 00:04Title
  • 00:36Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells
  • 03:10Incubation of the Transfected BW Cells with Targets
  • 03:32ELISA
  • 04:38Results: BW Reporter System Demonstrated
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions.

Tags

Keywords: BW Reporter SystemReceptor-ligand InteractionsLigandEndogenous LigandTransfectionPcDNA3 PlasmidSodium AcetateEthanol PrecipitationElectroporationG418 Selection96-well PlateELISA

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