JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
基于滴瓣的成年哺乳动物组织单细胞转录瘤
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
Please note that all translations are automatically generated.
Click here for the English version.
基于滴瓣的成年哺乳动物组织单细胞转录瘤
DOI:
10.3791/58709-v
•
10:12 min
•
January 10, 2019
•
Jo Anne Stratton*
1,2,3
,
Sarthak Sinha*
2
,
Wisoo Shin
,
Elodie Labit
,
Tak-Ho Chu
4
,
Prajay T. Shah
,
Rajiv Midha
4
,
Jeff Biernaskie
2,3
1
Hotchkiss Brain Institute
,
University of Calgary
,
2
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine
,
University of Calgary
,
3
Alberta Children’s Hospital Research Institute
,
University of Calgary
,
4
Department of Clinical Neurosciences, Cumming School of Medicine
,
University of Calgary
Chapters
00:04
Title
00:56
Dissociating Tissue (Day 1)
03:46
Isolating Viable and Healthy Cells (Day 1)
04:46
GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
06:17
NCBI’s GEO and SRA Submissions
07:34
Results: Bioinformatics Analysis of Single Cell Transcriptomics
09:35
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了为基于液滴、高通量的单细胞 rna-seq 制剂准备健康的成年哺乳动物单细胞所需的一般过程和质量控制检查。还提供了测序参数、读取对齐和下游单细胞生物信息学分析。
Tags
Single Cell Transcriptomics
Droplet Barcoding
Cell Isolation
Gene Expression
Functional Dynamics
Skin
Sciatic Nerve
Collagenase
DNAse
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
游离成年哺乳动物元DNA的细胞核内内微量注射
剖析器官的成年斑马鱼
在哺乳动物细胞的RNAi筛选使命esiRNA
成年小鼠心肌细胞的单细胞转录谱
在哺乳动物细胞中细胞周期位置分析
中血红素的合成水平在哺乳动物细胞中测量
高效的哺乳动物细胞表达和细胞外糖蛋白的结晶一步纯化
哺乳动物细胞系的厌氧生长与维持
Y-27632 丰富成人皮肤组织中人类黑色素细胞的产量
单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能
Read Article