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Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær
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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær

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6,672 Views

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09:23 min

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November 28, 2018

DOI:

10.3791/58748-v

DOI:
10.3791/58748-v

•

09:23 min
November 28, 2018

•

1 Views
, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Summary

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Denne artikel beskriver protokoller for at vurdere effekten af fluorescerende proteiner på en sammenlægning og toksicitet af fejlfoldede polyglutamin udvidelse til hurtig evaluering af en nyligt uncharacterized fluorescerende proteiner inden for rammerne af fluorescerende journalister.

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Cite this Article

Jiang, Y., Di Gregorio, S., Albakri, M. B., Duennwald, M. L., Lajoie, P. Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast. J. Vis. Exp. (141), e58748, doi:10.3791/58748 (2018).

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