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荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响
JoVE Journal
Biology
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JoVE Journal Biology
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
DOI:

09:23 min

November 28, 2018

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Chapters

  • 00:04Title
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

Automatic Translation

本文介绍了评估荧光蛋白对错误折叠的多谷氨酰胺扩张的聚集和毒性的影响的协议, 以便在荧光记者的背景下快速评估一种新的无特征荧光蛋白。

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