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Biology
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荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响
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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
Please note that all translations are automatically generated.
Click here for the English version.
荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响
DOI:
10.3791/58748-v
•
09:23 min
•
November 28, 2018
•
Yuwei Jiang
,
Sonja Di Gregorio
,
Maram B. Albakri
,
Martin L. Duennwald
2
,
Patrick Lajoie
1
Department of Anatomy and Cell Biology
,
University of Western Ontario
,
2
Department of Pathology and Laboratory Medicine
,
University of Western Ontario
Chapters
00:04
Title
00:40
Preparing Cell Cultures
02:25
Spotting Assay
03:36
Quantification of Cell Growth in Liquid Culture
04:49
Fluorescent Microscopy
05:32
Dot Blot
07:26
Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了评估荧光蛋白对错误折叠的多谷氨酰胺扩张的聚集和毒性的影响的协议, 以便在荧光记者的背景下快速评估一种新的无特征荧光蛋白。
Tags
Fluorescent Proteins
Fusion Partners
Polyglutamine Toxicity
Yeast
HTT Exon One
25Q
72Q
Monomeric Superfolder GFP
GAL1 Promoter
PolyQ Fusions
Optical Density
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