Journal
/
Biology
/
用化学干燥进行扫描电镜 (sem) 形态分析的原核生物和真核生物的制备
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
Please note that all translations are automatically generated. Click here for the English version.

用化学干燥进行扫描电镜 (sem) 形态分析的原核生物和真核生物的制备

DOI:
10.3791/58761-v

•

09:58 min

•

January 07, 2019

•

, , , , ,
1Department of Biology,Central Michigan University

Chapters

  • 00:04Title
  • 00:45Fixation, Washing and Dehydration of Cyanobacteria and Euglenoids
  • 02:36Drying and Mounting
  • 03:54Fixation, Washing and Dehydration of Drosophila
  • 04:55Drosophila Drying and Mounting
  • 06:48Sputter Coating and Imaging
  • 07:20Results: Morphological Analysis of Different Organisms Using SEM Images
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

扫描电镜分析是帮助物种鉴定或表型鉴别的有效方法。该协议描述了检查三种具有代表性的生物体的具体形态细节的方法, 并将广泛适用于检查许多组织和组织类型的特征。

Tags

Scanning Electron MicroscopySEMChemical DryingHexamethyldisilazaneT-butyl AlcoholProkaryotic OrganismsEukaryotic OrganismsCyanobacteriaEuglenoidsFiltrationDehydrationFixationPhosphate BufferEthanol SeriesChemical Drying

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

电子显微镜对胚胎的制备果蝇胚胎心管

一个标准化的方法肝窦内皮细胞的分析及其窗孔用扫描电子显微镜

扫描浸软组织的电子显微镜可视化的细胞外基质

通过低温扫描电子显微镜研究光合膜内冻结，割断叶片组织的超分子组织

扫描电子显微镜（SEM）协议有问题的工厂，卵菌和真菌样本

准备厚生物样品的观察扫描透射电子断层扫描

透射电镜下体的样品制备与成像

透射电镜的小型化样品制备方法

高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备

阵列断层扫描工作流程，用于使用扫描电子显微镜有针对性地采集体积信息

Read Article