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JoVE Journal Neuroscience
Two-photon Imaging d’Attraction des microglies processus vers l’ATP ou la sérotonine cérébrale aiguë tranches
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Two-photon Imaging d’Attraction des microglies processus vers l’ATP ou la sérotonine cérébrale aiguë tranches

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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La microglie, les résidents cellules immunitaires du cerveau, répondre rapidement avec des changements morphologiques aux modifications de leur environnement. Ce protocole décrit comment utiliser la microscopie biphotonique à étudier l’attraction des microglies processus vers la sérotonine ou l’ATP en tranches cérébrale aiguë des souris.

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Neurosciences numéro 143 microglie microscopie multiphotonique tranches cérébrale aiguë chimioattraction motilité morphologie sérotonine ATP imagerie live time-lapse imagerie
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