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To-foton billeddannelse af Microglial processer tiltrækning mod ATP eller Serotonin i akut hjernen skiver
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
DOI:

07:27 min

January 31, 2019

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Chapters

  • 00:04Title
  • 00:42Dissection Area Preparation
  • 01:51Brain Slicing
  • 03:112-photon Live Imaging
  • 04:12Compound Application
  • 06:14Results: Microglial Response Analyses
  • 07:05Conclusion

Summary

Automatic Translation

Mikroglia, hjernen, bosiddende immunceller reagere hurtigt med morfologiske ændringer til ændringer i deres miljø. Denne protokol beskriver, hvordan du bruger to-foton mikroskopi til at studere tiltrækning af microglial processer mod serotonin eller ATP i akut hjernen skiver af mus.

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