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基于多色流式细胞仪的 t 细胞线粒体和溶酶体的定量
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
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基于多色流式细胞仪的 t 细胞线粒体和溶酶体的定量

DOI:
10.3791/58844-v

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06:22 min

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January 09, 2019

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, , ,
1Institute of Microbiology and Immunology,National Yang-Ming University

Chapters

  • 00:04Title
  • 00:46Mitochondria and Lysosome Labeling
  • 02:27Flow Cytometric Analysis
  • 04:31Results: Representative Mitochondria and Lysosome Detection
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

本文说明了一种对活细胞中线粒体或溶酶体进行量化的有力方法。将溶酶体或线粒体特异性染料与针对表面标记物的荧光共轭抗体结合使用, 可以通过使用多色流式细胞术。

Tags

Flow CytometryMitochondriaLysosomesT CellsOrganelle QuantificationImmuno-metabolismCell LabelingCell StainingFluorescence-activated Cell Sorting (FACS)

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