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JoVE Journal
Immunology and Infection

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Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler
 
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Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler

Article DOI: 10.3791/58844-v
January 9th, 2019

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Summary January 9th, 2019

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Denne artikel illustrerer en kraftfuld metode til at anslå mitokondrier eller lysosomer i levende celler. Kombinationen af lysosomet eller mitokondrier specifikke farvestoffer med fluorescently konjugerede antistoffer mod celleoverflademarkører giver mulighed for kvantificering af disse organeller i blandet cellepopulationer, som primær celler høstet fra vævsprøver, ved hjælp af flerfarvet flowcytometri.

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