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Journal
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Biochemistry
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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
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Biochemistry
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JoVE Journal
Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Please note that all translations are automatically generated.
Click here for the English version.
放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
DOI:
10.3791/58952-v
•
08:59 min
•
February 12, 2019
•
Nicholas McCaul
2
,
Hui Ying Yeoh
,
Guus van Zadelhoff
,
Naomi Lodder
,
Bertrand Kleizen
,
Ineke Braakman
1
Cellular Protein Chemistry
,
Utrecht University
,
2
Program in Cellular and Molecular Medicine
,
Boston Children’s Hospital
Chapters
00:04
Title
01:05
Pulse Chase for Adherent Cells
04:07
Pulse Chase for Suspension Cells
05:03
Immunoprecipitation
06:16
Analysis of the Radioactive Pulse Chase Results
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一种一般脉冲追逐方法的协议, 该方法允许在活细胞中对蛋白质的折叠、迁移和降解进行动力学分析。
Tags
Protein Folding
Protein Transport
Protein Degradation
Radioactive Pulse Chase
35S-labeled Methionine And Cysteine
Protein Biosynthesis
Co- And Post-translational Modifications
Disulfide Bond Formation
Acetylation
Cell Culture
Transfection
Starvation Medium
Pulse Solution
Chase Medium
Stop Buffer
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