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一种从人类死后小脑中的激光捕获微解剖 purkinje 细胞中分离高质量 rna 的不锈钢协议
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A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum
Please note that all translations are automatically generated.
Click here for the English version.
一种从人类死后小脑中的激光捕获微解剖 purkinje 细胞中分离高质量 rna 的不锈钢协议
DOI:
10.3791/58953-v
•
09:04 min
•
January 17, 2019
•
Regina T. Martuscello
,
Elan D. Louis
3,4
,
Phyllis L. Faust
1
Department of Pathology and Cell Biology
,
Columbia University
,
2
Division of Movement Disorders, Department of Neurology
,
Yale University
,
3
Department of Chronic Disease Epidemiology, Yale School of Public Health
,
Yale University
,
4
Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine
,
Yale University
Chapters
00:04
Title
00:33
Prepare Cryostat for Tissue Sectioning
01:51
Sectioning Tissue
03:50
Fixing Tissue/Stain-less Visualization
04:47
Laser Capture Microdissection of Purkinje Cells
06:09
Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议采用无污渍的方法, 通过激光捕获微解剖, 将新鲜冷冻组织中的 purkinje 细胞从人类死后的小脑中观察和分离。该协议的目的是为 rna 测序生成足够数量的高质量 rna。
Tags
RNA Isolation
Laser Capture Microdissection
Purkinje Cells
Post-mortem Human Cerebellum
Stain-free Visualization
Cryostat
OCT
RNase Decontamination
Temperature Control
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