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一种改进的高通量呼吸道合胞病毒 (rsv) 微中和检测方法
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JoVE Journal
Medicine
An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Please note that all translations are automatically generated.
Click here for the English version.
一种改进的高通量呼吸道合胞病毒 (rsv) 微中和检测方法
DOI:
10.3791/59025-v
•
09:14 min
•
January 26, 2019
•
Lien Anh Ha Do
2
,
Reuben Tse
,
Jordan Nathanielsz
,
Jeremy Anderson
,
Darren Suryawijaya Ong
,
Keith Chappell
,
Kim Mulholland
2,4
,
Paul V. Licciardi
2
1
Murdoch Children’s Research Institute
,
2
Department of Paediatrics
,
University of Melbourne
,
3
School of Chemistry and Molecular Bioscience
,
University of Queensland
,
4
London School of Hygiene and Tropical Medicine
Chapters
00:04
Title
00:48
Assay Setup
05:23
Assay Development
07:40
Results: A High-throughput Image-based RSV Neutralization Assay
08:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究描述了一种高吞吐量
、基于图像的微中和方法, 以确定呼吸合胞病毒 (rsv) 特异性中和抗体的滴度。这种检测格式已在不同的样品类型上进行了测试。
Tags
Respiratory Syncytial Virus
RSV
Micro-neutralization Assay
Neutralizing Antibodies
High Throughput
Imaging-based
A549 Cells
Serum Dilution
Reference Serum
Sample Preparation
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